Efficient purification of bromoviruses by ultrafiltration.

نویسندگان

  • Jean-Philippe Michel
  • Mari Gingery
  • Laurence Lavelle
چکیده

Ultrafiltration using polyethersulfone-membranes was evaluated as an efficient and preferred method for purifying Cowpea Chlorotic Mottle Virus (CCMV). Cesium chloride (CsCl) ultracentrifugation and ultrafiltration protocols are described, and comparative UV-spectroscopic and electron micrograph results are presented. CCMV purified by ultrafiltration are shown to be equivalent to CCMV purified by ultracentrifugation, while reducing purification time by two days and avoiding the need for expensive capital overheads such as ultracentrifuges, rotors and toxic CsCl chemical waste.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Efficient Recovery of Hyaluronic Acid from Highly Viscous Culture Broth

15 An efficient method for the separation and recovery of Hyaluronic Acid (HA) from highly viscous broth was developed. In this strategy, the separation of HA from viscous culture broth and purification by optimizing different parameters, viz., temperature, pH, treatment with activated charcoal, were included. In addition, concentrating the HA solution using ultrafiltration facilitated the redu...

متن کامل

Comparison of Ultrafiltration and Size-exclusion Chromatography Performances for Proteins Desalination

Tampereen ammattikorkeakoulu Tampere University of Applied Sciences Paper, Textile and Chemical Engineering Arina Akhmetova: Comparison of ultrafiltration and size-exclusion chromatography performances for proteins desalination Bachelor's thesis 41 pages November 2014 Protein purification is a part of biotechnology. Its application can vary from laboratory researches to industrial production. N...

متن کامل

Rapid and Efficient Purification Method for Small, Hydrophobic, Cationic Bacteriocins: Purification of Lactococcin B and Pediocin PA-1.

The bacteriocins lactococcin B and pediocin PA-1 were purified by ethanol precipitation, preparative isoelectric focusing, and ultrafiltration. The procedure reproducibly leads to high final yields in comparison to the generally low yields obtained by column chromatography. Specifically, during isoelectric focusing no loss of activity occurs. The method, in general, should be applicable to smal...

متن کامل

Preliminary Study of the Efficiency of Ultrafiltration Treatment of Poultry Slaughterhouse Wastewater

YORDANOV, D., 2010. Preliminary study of the efficiency of ultrafiltration treatment of poultry slaughterhouse wastewater. Bulg. J. Agric. Sci., 16: 700-704 Poultry slaughterhouses use relatively high amount of water with an average consumption of 26.5 L/bird during primary and secondary processing of live birds to meat. The used water contains proteins, fats, carbohydrates from meat, blood, sk...

متن کامل

Performance Evaluation of Ceramic Membrane on Ultrafiltration and Diafiltration Modes for Efficient Recovery of Whey Protein

The recovery of high-quality, valuable end products from cheese whey is a very important industrial process. The aim of this study is to improve the recovered protein content of concentrate stream in cheese whey. The experiments were conducted by using ceramic ultrafiltration (UF) membrane followed by diafiltration (DF) process with a 15kDa molecular weight cut-off (MWCO). The volume concentrat...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of virological methods

دوره 122 2  شماره 

صفحات  -

تاریخ انتشار 2004